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cd4 cd8α cd19 cd56 cells  (Miltenyi Biotec)


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    Miltenyi Biotec cd4 cd8α cd19 cd56 cells
    Cd4 Cd8α Cd19 Cd56 Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd56+cells/PSA-NCAM+Antibody%2C+anti-human%2Fmouse%2Frat/pm42009279-279-0-14
    Average 96 stars, based on 42 article reviews
    cd4 cd8α cd19 cd56 cells - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Allogeneic CD56 + cell-based immunotherapy in a patient with Fanconi anemia developing acute myeloid leukemia.
    Article Snippet: .. CD56+ activation For activation, enriched CD56+ cells were incubated overnight in NKMACS® GMP medium (Miltenyi Biotech, Germany) supplemented with 5% fetal bovine serum (FBS) (HyCloneTM, United States), 1% NK MACS supplement, and 20 ng/mL of MACS® GMP-grade human IL-15 (Miltenyi Biotech, Germany). .. Following incubation, cells were 1 3 79 Page 2 of 7 Journal of Cancer Research and Clinical Oncology (2026) 152:79 washed with PBS/EDTA (Miltenyi Biotech, Germany) and prepared for infusion or cryopreservation.

    Incubation:

    Article Title: Allogeneic CD56 + cell-based immunotherapy in a patient with Fanconi anemia developing acute myeloid leukemia.
    Article Snippet: .. CD56+ activation For activation, enriched CD56+ cells were incubated overnight in NKMACS® GMP medium (Miltenyi Biotech, Germany) supplemented with 5% fetal bovine serum (FBS) (HyCloneTM, United States), 1% NK MACS supplement, and 20 ng/mL of MACS® GMP-grade human IL-15 (Miltenyi Biotech, Germany). .. Following incubation, cells were 1 3 79 Page 2 of 7 Journal of Cancer Research and Clinical Oncology (2026) 152:79 washed with PBS/EDTA (Miltenyi Biotech, Germany) and prepared for infusion or cryopreservation.

    Article Title: Allogeneic CD56 + cell-based immunotherapy in a patient with Fanconi anemia developing acute myeloid leukemia.
    Article Snippet: .. Before and after incubation with IL-15, the phenotype of CD56+ cells was evaluated by flow cytometry (MACS QUNT 10, Miltenyi Biotech, Germany). ..

    Magnetic Cell Separation:

    Article Title: Allogeneic CD56 + cell-based immunotherapy in a patient with Fanconi anemia developing acute myeloid leukemia.
    Article Snippet: .. CD56+ activation For activation, enriched CD56+ cells were incubated overnight in NKMACS® GMP medium (Miltenyi Biotech, Germany) supplemented with 5% fetal bovine serum (FBS) (HyCloneTM, United States), 1% NK MACS supplement, and 20 ng/mL of MACS® GMP-grade human IL-15 (Miltenyi Biotech, Germany). .. Following incubation, cells were 1 3 79 Page 2 of 7 Journal of Cancer Research and Clinical Oncology (2026) 152:79 washed with PBS/EDTA (Miltenyi Biotech, Germany) and prepared for infusion or cryopreservation.

    Article Title: Feeder-free ex vivo expansion of cord blood-derived natural killer cells for enhanced proliferation and functional maturation.
    Article Snippet: Prior to CD56+ cell isolation by magnetic-activated cell sorting (MACS), a preenrichment characterization was performed to determine the percentage of CD3CD56+ cells by flow cytometry (CD3 PerCP-Cy5.5 (BioLegend) and CD56 PE (BioLegend)). .. CD56+ cells were isolated using MACS with CD56 MicroBeads (Miltenyi Biotec), following the manufacturer instructions, and MACS efficiency was calculated using Equation 1. .. Briefly, MNC(CB) were resuspended in PBS supplemented with 0.5% (v/v) bovine serum albumin (Sigma-Aldrich) and 2 mM EDTA (MACS Buffer).

    Article Title: Allogeneic CD56 + cell-based immunotherapy in a patient with Fanconi anemia developing acute myeloid leukemia.
    Article Snippet: .. Before and after incubation with IL-15, the phenotype of CD56+ cells was evaluated by flow cytometry (MACS QUNT 10, Miltenyi Biotech, Germany). ..

    Cell Culture:

    Article Title: Astaxanthin improves myogenicity of aged skeletal muscle progenitor cells in a sexually dimorphic manner
    Article Snippet: .. After 24 h, the supernatant containing unattached myoblasts was transferred to a new T75 flask, and cultured until cells reached 70–80% confluence, with media changes performed every 48 h. We performed magnetic assisted cell sorting to isolate CD56+ cells, according to the manufacturer’s protocol (130-097-042, Miltenyi Biotec). ..

    FACS:

    Article Title: Astaxanthin improves myogenicity of aged skeletal muscle progenitor cells in a sexually dimorphic manner
    Article Snippet: .. After 24 h, the supernatant containing unattached myoblasts was transferred to a new T75 flask, and cultured until cells reached 70–80% confluence, with media changes performed every 48 h. We performed magnetic assisted cell sorting to isolate CD56+ cells, according to the manufacturer’s protocol (130-097-042, Miltenyi Biotec). ..

    Article Title: Astaxanthin improves myogenicity of aged skeletal muscle progenitor cells in a sexually dimorphic manner.
    Article Snippet: .. We performed magnetic assisted cell sorting to isolate CD56+ cells, according to the manufacturer’s protocol (130-097-042, Miltenyi Biotec). ..

    Magnetic Beads:

    Article Title: Revealing an enhanced cytotoxic immune microenvironment at the human maternal–fetal interface in preeclampsia
    Article Snippet: .. dNK cells were enriched from first-trimester decidua immune cells by removing dead cells(Miltenyi, Cat# 130–090–101, Germany) and collecting CD56 + cells using magnetic beads (Miltenyi, Cat# 130–050–401, Germany). .. Purified dNK cells were then used for flow cytometry, cytotoxicity assays, or cultured with 20 ng/ml IL-15 (PeproTech, Cat# AF-200–15).

    Immunomagnetic Separation:

    Article Title: Methods of selecting T cell line and donor thereof for adoptive cellular therapy
    Article Snippet: .. Generation of CMVpp65 Specific T-Cells: T-cells were enriched from peripheral blood lymphocytes separated from the PBMCs by depletion of adherent monocytes followed by depletion of natural killer cells by using immunomagnetic separation of CD56+ cells with immunomagnetic CD56 precoated microbeads (Miltenyi Biotech Inc.). ..

    Isolation:

    Article Title: Feeder-free ex vivo expansion of cord blood-derived natural killer cells for enhanced proliferation and functional maturation.
    Article Snippet: Prior to CD56+ cell isolation by magnetic-activated cell sorting (MACS), a preenrichment characterization was performed to determine the percentage of CD3CD56+ cells by flow cytometry (CD3 PerCP-Cy5.5 (BioLegend) and CD56 PE (BioLegend)). .. CD56+ cells were isolated using MACS with CD56 MicroBeads (Miltenyi Biotec), following the manufacturer instructions, and MACS efficiency was calculated using Equation 1. .. Briefly, MNC(CB) were resuspended in PBS supplemented with 0.5% (v/v) bovine serum albumin (Sigma-Aldrich) and 2 mM EDTA (MACS Buffer).

    Flow Cytometry:

    Article Title: Allogeneic CD56 + cell-based immunotherapy in a patient with Fanconi anemia developing acute myeloid leukemia.
    Article Snippet: .. Before and after incubation with IL-15, the phenotype of CD56+ cells was evaluated by flow cytometry (MACS QUNT 10, Miltenyi Biotech, Germany). ..



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    Single-cell transcriptomics analysis reveals distinct cellular compositions in PE patients and controls. ( A ) Schematic workflow for single-cell transcriptomic analysis of sorted CD45⁺ immune cells from decidua, including three from preeclampsia (PE) patients and three from healthy controls. ( B ) Dot plot illustrating the expression levels and relative proportions of specific marker genes across immune cell clusters. ( C , D , E ) UMAP visualization depicting 7 major immune cell clusters and 22 subclusters within decidua CD45⁺ cells from 6 samples. ( F ) Feature plot illustrating the expression of selected marker genes across different clusters. ( G ) Heatmap depicting the top 10 DEGs in each of the 22 clusters. ( H ) Bar plot showing the proportion of each immune cell cluster across individual samples. ( I ) Representative immunostaining images of NK cells <t>(CD56),</t> T cells (CD3), and macrophages (CD68) in decidual tissue from PE and control samples. Scale bars: 50 µm
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    Single-cell transcriptomics analysis reveals distinct cellular compositions in PE patients and controls. ( A ) Schematic workflow for single-cell transcriptomic analysis of sorted CD45⁺ immune cells from decidua, including three from preeclampsia (PE) patients and three from healthy controls. ( B ) Dot plot illustrating the expression levels and relative proportions of specific marker genes across immune cell clusters. ( C , D , E ) UMAP visualization depicting 7 major immune cell clusters and 22 subclusters within decidua CD45⁺ cells from 6 samples. ( F ) Feature plot illustrating the expression of selected marker genes across different clusters. ( G ) Heatmap depicting the top 10 DEGs in each of the 22 clusters. ( H ) Bar plot showing the proportion of each immune cell cluster across individual samples. ( I ) Representative immunostaining images of NK cells <t>(CD56),</t> T cells (CD3), and macrophages (CD68) in decidual tissue from PE and control samples. Scale bars: 50 µm
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    Single-cell transcriptomics analysis reveals distinct cellular compositions in PE patients and controls. ( A ) Schematic workflow for single-cell transcriptomic analysis of sorted CD45⁺ immune cells from decidua, including three from preeclampsia (PE) patients and three from healthy controls. ( B ) Dot plot illustrating the expression levels and relative proportions of specific marker genes across immune cell clusters. ( C , D , E ) UMAP visualization depicting 7 major immune cell clusters and 22 subclusters within decidua CD45⁺ cells from 6 samples. ( F ) Feature plot illustrating the expression of selected marker genes across different clusters. ( G ) Heatmap depicting the top 10 DEGs in each of the 22 clusters. ( H ) Bar plot showing the proportion of each immune cell cluster across individual samples. ( I ) Representative immunostaining images of NK cells <t>(CD56),</t> T cells (CD3), and macrophages (CD68) in decidual tissue from PE and control samples. Scale bars: 50 µm
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    Single-cell transcriptomics analysis reveals distinct cellular compositions in PE patients and controls. ( A ) Schematic workflow for single-cell transcriptomic analysis of sorted CD45⁺ immune cells from decidua, including three from preeclampsia (PE) patients and three from healthy controls. ( B ) Dot plot illustrating the expression levels and relative proportions of specific marker genes across immune cell clusters. ( C , D , E ) UMAP visualization depicting 7 major immune cell clusters and 22 subclusters within decidua CD45⁺ cells from 6 samples. ( F ) Feature plot illustrating the expression of selected marker genes across different clusters. ( G ) Heatmap depicting the top 10 DEGs in each of the 22 clusters. ( H ) Bar plot showing the proportion of each immune cell cluster across individual samples. ( I ) Representative immunostaining images of NK cells <t>(CD56),</t> T cells (CD3), and macrophages (CD68) in decidual tissue from PE and control samples. Scale bars: 50 µm
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    Image Search Results


    Single-cell transcriptomics analysis reveals distinct cellular compositions in PE patients and controls. ( A ) Schematic workflow for single-cell transcriptomic analysis of sorted CD45⁺ immune cells from decidua, including three from preeclampsia (PE) patients and three from healthy controls. ( B ) Dot plot illustrating the expression levels and relative proportions of specific marker genes across immune cell clusters. ( C , D , E ) UMAP visualization depicting 7 major immune cell clusters and 22 subclusters within decidua CD45⁺ cells from 6 samples. ( F ) Feature plot illustrating the expression of selected marker genes across different clusters. ( G ) Heatmap depicting the top 10 DEGs in each of the 22 clusters. ( H ) Bar plot showing the proportion of each immune cell cluster across individual samples. ( I ) Representative immunostaining images of NK cells (CD56), T cells (CD3), and macrophages (CD68) in decidual tissue from PE and control samples. Scale bars: 50 µm

    Journal: Cell Biology and Toxicology

    Article Title: Revealing an enhanced cytotoxic immune microenvironment at the human maternal–fetal interface in preeclampsia

    doi: 10.1007/s10565-026-10170-7

    Figure Lengend Snippet: Single-cell transcriptomics analysis reveals distinct cellular compositions in PE patients and controls. ( A ) Schematic workflow for single-cell transcriptomic analysis of sorted CD45⁺ immune cells from decidua, including three from preeclampsia (PE) patients and three from healthy controls. ( B ) Dot plot illustrating the expression levels and relative proportions of specific marker genes across immune cell clusters. ( C , D , E ) UMAP visualization depicting 7 major immune cell clusters and 22 subclusters within decidua CD45⁺ cells from 6 samples. ( F ) Feature plot illustrating the expression of selected marker genes across different clusters. ( G ) Heatmap depicting the top 10 DEGs in each of the 22 clusters. ( H ) Bar plot showing the proportion of each immune cell cluster across individual samples. ( I ) Representative immunostaining images of NK cells (CD56), T cells (CD3), and macrophages (CD68) in decidual tissue from PE and control samples. Scale bars: 50 µm

    Article Snippet: dNK cells were enriched from first-trimester decidua immune cells by removing dead cells(Miltenyi, Cat# 130–090–101, Germany) and collecting CD56 + cells using magnetic beads (Miltenyi, Cat# 130–050–401, Germany).

    Techniques: Single-cell Transcriptomics, Single Cell, Expressing, Marker, Immunostaining, Control

    Decidua NK cells in PE have a specific developmental trajectory along with enhanced cytotoxicity which may lead to trophoblast cells apoptosis. ( A ) UMAP visualization of decidua NK (dNK) cells, identifying four major subpopulations. ( B ) Bar plot showing the proportions of the four dNK subclusters in PE and Control samples. ( C ) Violin plot comparing the expression levels of individual genes in dNK subclusters between PE and Control samples. ( D ) UMAP visualization revealing two distinct developmental trajectories of dNK cells. ( E ) UMAP visualization showing dNK cells from two different groups. ( F ) Curve plots showing dynamic expression changes of selected genes along pseudotime in the two differentiation pathways. ( G ) Pseudocolor plots and bar plots comparing the apoptosis rate of HTR8-SVneo cell cocultured with dNK (-K562) or dNK (+K562), (n=3). Data are expressed as mean ± SD, unpaired t test was used for comparisons among two groups. Statistical significance: * p < 0.05. (H) Representative immunostaining images of extravillous trophoblast cells (HLA-G) and dNK cells (CD56) from PE and control samples. Scale bars: 100 µm

    Journal: Cell Biology and Toxicology

    Article Title: Revealing an enhanced cytotoxic immune microenvironment at the human maternal–fetal interface in preeclampsia

    doi: 10.1007/s10565-026-10170-7

    Figure Lengend Snippet: Decidua NK cells in PE have a specific developmental trajectory along with enhanced cytotoxicity which may lead to trophoblast cells apoptosis. ( A ) UMAP visualization of decidua NK (dNK) cells, identifying four major subpopulations. ( B ) Bar plot showing the proportions of the four dNK subclusters in PE and Control samples. ( C ) Violin plot comparing the expression levels of individual genes in dNK subclusters between PE and Control samples. ( D ) UMAP visualization revealing two distinct developmental trajectories of dNK cells. ( E ) UMAP visualization showing dNK cells from two different groups. ( F ) Curve plots showing dynamic expression changes of selected genes along pseudotime in the two differentiation pathways. ( G ) Pseudocolor plots and bar plots comparing the apoptosis rate of HTR8-SVneo cell cocultured with dNK (-K562) or dNK (+K562), (n=3). Data are expressed as mean ± SD, unpaired t test was used for comparisons among two groups. Statistical significance: * p < 0.05. (H) Representative immunostaining images of extravillous trophoblast cells (HLA-G) and dNK cells (CD56) from PE and control samples. Scale bars: 100 µm

    Article Snippet: dNK cells were enriched from first-trimester decidua immune cells by removing dead cells(Miltenyi, Cat# 130–090–101, Germany) and collecting CD56 + cells using magnetic beads (Miltenyi, Cat# 130–050–401, Germany).

    Techniques: Control, Expressing, Immunostaining